Review



cfps kit  (Bioneer Corporation)

 
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    Structured Review

    Bioneer Corporation cfps kit
    SDS-PAGE and Western blot analysis of P. insidiosum 's synthesized proteins. Nineteen coding sequences of the recruited target proteins S01-S21 (except S03 and S04) serve as a <t>protein</t> <t>synthesis</t> template in the <t>CFPS</t> reaction, and the resulting products are checked for their presence using SDS-PAGE stained with Coomassie blue dye ( A ) and their immunoreactivity using Western blot analysis probed against the mouse anti-6x-His tag antibody ( B ). Green fluorescent protein (GFP) is a positive control for the CFPS reaction. An arrowhead indicates a target synthesized protein. The molecular weight markers (M) are shown on the left of each figure. The original unprocessed images of are available as supplementary files: Original_Fig2A_S01-02_S11-S14_GFP.tif, Original_Fig2A_S05-06.tif, Original_Fig2A_S07-10.tif, Original_Fig2A_S015-21.tif, Original_Fig2B_S01-S02_S11-S14_GFP.tif, Original_Fig2B_S05-06.tif, Original_Fig2B_S07-10.tif, and Original_Fig2B_S015-21.tif.
    Cfps Kit, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfps+kit/pmc11959653-200-23-26?v=Bioneer+Corporation
    Average 86 stars, based on 1 article reviews
    cfps kit - by Bioz Stars, 2026-07
    86/100 stars

    Images

    1) Product Images from "Immunoproteomic approach identifies a putative virulence chaperone DnaK protein as a candidate diagnostic marker and therapeutic target for Pythium insidiosum infection"

    Article Title: Immunoproteomic approach identifies a putative virulence chaperone DnaK protein as a candidate diagnostic marker and therapeutic target for Pythium insidiosum infection

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2025.e42487

    SDS-PAGE and Western blot analysis of P. insidiosum 's synthesized proteins. Nineteen coding sequences of the recruited target proteins S01-S21 (except S03 and S04) serve as a protein synthesis template in the CFPS reaction, and the resulting products are checked for their presence using SDS-PAGE stained with Coomassie blue dye ( A ) and their immunoreactivity using Western blot analysis probed against the mouse anti-6x-His tag antibody ( B ). Green fluorescent protein (GFP) is a positive control for the CFPS reaction. An arrowhead indicates a target synthesized protein. The molecular weight markers (M) are shown on the left of each figure. The original unprocessed images of are available as supplementary files: Original_Fig2A_S01-02_S11-S14_GFP.tif, Original_Fig2A_S05-06.tif, Original_Fig2A_S07-10.tif, Original_Fig2A_S015-21.tif, Original_Fig2B_S01-S02_S11-S14_GFP.tif, Original_Fig2B_S05-06.tif, Original_Fig2B_S07-10.tif, and Original_Fig2B_S015-21.tif.
    Figure Legend Snippet: SDS-PAGE and Western blot analysis of P. insidiosum 's synthesized proteins. Nineteen coding sequences of the recruited target proteins S01-S21 (except S03 and S04) serve as a protein synthesis template in the CFPS reaction, and the resulting products are checked for their presence using SDS-PAGE stained with Coomassie blue dye ( A ) and their immunoreactivity using Western blot analysis probed against the mouse anti-6x-His tag antibody ( B ). Green fluorescent protein (GFP) is a positive control for the CFPS reaction. An arrowhead indicates a target synthesized protein. The molecular weight markers (M) are shown on the left of each figure. The original unprocessed images of are available as supplementary files: Original_Fig2A_S01-02_S11-S14_GFP.tif, Original_Fig2A_S05-06.tif, Original_Fig2A_S07-10.tif, Original_Fig2A_S015-21.tif, Original_Fig2B_S01-S02_S11-S14_GFP.tif, Original_Fig2B_S05-06.tif, Original_Fig2B_S07-10.tif, and Original_Fig2B_S015-21.tif.

    Techniques Used: SDS Page, Western Blot, Synthesized, Staining, Positive Control, Molecular Weight



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    SDS-PAGE and Western blot analysis of P. insidiosum 's synthesized proteins. Nineteen coding sequences of the recruited target proteins S01-S21 (except S03 and S04) serve as a <t>protein</t> <t>synthesis</t> template in the <t>CFPS</t> reaction, and the resulting products are checked for their presence using SDS-PAGE stained with Coomassie blue dye ( A ) and their immunoreactivity using Western blot analysis probed against the mouse anti-6x-His tag antibody ( B ). Green fluorescent protein (GFP) is a positive control for the CFPS reaction. An arrowhead indicates a target synthesized protein. The molecular weight markers (M) are shown on the left of each figure. The original unprocessed images of are available as supplementary files: Original_Fig2A_S01-02_S11-S14_GFP.tif, Original_Fig2A_S05-06.tif, Original_Fig2A_S07-10.tif, Original_Fig2A_S015-21.tif, Original_Fig2B_S01-S02_S11-S14_GFP.tif, Original_Fig2B_S05-06.tif, Original_Fig2B_S07-10.tif, and Original_Fig2B_S015-21.tif.
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    SDS-PAGE and Western blot analysis of P. insidiosum 's synthesized proteins. Nineteen coding sequences of the recruited target proteins S01-S21 (except S03 and S04) serve as a <t>protein</t> <t>synthesis</t> template in the <t>CFPS</t> reaction, and the resulting products are checked for their presence using SDS-PAGE stained with Coomassie blue dye ( A ) and their immunoreactivity using Western blot analysis probed against the mouse anti-6x-His tag antibody ( B ). Green fluorescent protein (GFP) is a positive control for the CFPS reaction. An arrowhead indicates a target synthesized protein. The molecular weight markers (M) are shown on the left of each figure. The original unprocessed images of are available as supplementary files: Original_Fig2A_S01-02_S11-S14_GFP.tif, Original_Fig2A_S05-06.tif, Original_Fig2A_S07-10.tif, Original_Fig2A_S015-21.tif, Original_Fig2B_S01-S02_S11-S14_GFP.tif, Original_Fig2B_S05-06.tif, Original_Fig2B_S07-10.tif, and Original_Fig2B_S015-21.tif.
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    SDS-PAGE and Western blot analysis of P. insidiosum 's synthesized proteins. Nineteen coding sequences of the recruited target proteins S01-S21 (except S03 and S04) serve as a <t>protein</t> <t>synthesis</t> template in the <t>CFPS</t> reaction, and the resulting products are checked for their presence using SDS-PAGE stained with Coomassie blue dye ( A ) and their immunoreactivity using Western blot analysis probed against the mouse anti-6x-His tag antibody ( B ). Green fluorescent protein (GFP) is a positive control for the CFPS reaction. An arrowhead indicates a target synthesized protein. The molecular weight markers (M) are shown on the left of each figure. The original unprocessed images of are available as supplementary files: Original_Fig2A_S01-02_S11-S14_GFP.tif, Original_Fig2A_S05-06.tif, Original_Fig2A_S07-10.tif, Original_Fig2A_S015-21.tif, Original_Fig2B_S01-S02_S11-S14_GFP.tif, Original_Fig2B_S05-06.tif, Original_Fig2B_S07-10.tif, and Original_Fig2B_S015-21.tif.
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    Image Search Results


    SDS-PAGE and Western blot analysis of P. insidiosum 's synthesized proteins. Nineteen coding sequences of the recruited target proteins S01-S21 (except S03 and S04) serve as a protein synthesis template in the CFPS reaction, and the resulting products are checked for their presence using SDS-PAGE stained with Coomassie blue dye ( A ) and their immunoreactivity using Western blot analysis probed against the mouse anti-6x-His tag antibody ( B ). Green fluorescent protein (GFP) is a positive control for the CFPS reaction. An arrowhead indicates a target synthesized protein. The molecular weight markers (M) are shown on the left of each figure. The original unprocessed images of are available as supplementary files: Original_Fig2A_S01-02_S11-S14_GFP.tif, Original_Fig2A_S05-06.tif, Original_Fig2A_S07-10.tif, Original_Fig2A_S015-21.tif, Original_Fig2B_S01-S02_S11-S14_GFP.tif, Original_Fig2B_S05-06.tif, Original_Fig2B_S07-10.tif, and Original_Fig2B_S015-21.tif.

    Journal: Heliyon

    Article Title: Immunoproteomic approach identifies a putative virulence chaperone DnaK protein as a candidate diagnostic marker and therapeutic target for Pythium insidiosum infection

    doi: 10.1016/j.heliyon.2025.e42487

    Figure Lengend Snippet: SDS-PAGE and Western blot analysis of P. insidiosum 's synthesized proteins. Nineteen coding sequences of the recruited target proteins S01-S21 (except S03 and S04) serve as a protein synthesis template in the CFPS reaction, and the resulting products are checked for their presence using SDS-PAGE stained with Coomassie blue dye ( A ) and their immunoreactivity using Western blot analysis probed against the mouse anti-6x-His tag antibody ( B ). Green fluorescent protein (GFP) is a positive control for the CFPS reaction. An arrowhead indicates a target synthesized protein. The molecular weight markers (M) are shown on the left of each figure. The original unprocessed images of are available as supplementary files: Original_Fig2A_S01-02_S11-S14_GFP.tif, Original_Fig2A_S05-06.tif, Original_Fig2A_S07-10.tif, Original_Fig2A_S015-21.tif, Original_Fig2B_S01-S02_S11-S14_GFP.tif, Original_Fig2B_S05-06.tif, Original_Fig2B_S07-10.tif, and Original_Fig2B_S015-21.tif.

    Article Snippet: Up to 16 different proteins can be synthesized simultaneously using a second-round PCR product as a template for protein synthesis and a commercial CFPS kit from Bioneer with an automated protein synthesis machine from the same company.

    Techniques: SDS Page, Western Blot, Synthesized, Staining, Positive Control, Molecular Weight